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Lucerna-Chem AG
Abendweg 18
CH - 6006 Luzern
Switzerland

 

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The LUMINEX Assay Principle

The LUMINEX system is based on laboratory multianalyte profiling (LabMAP™) technology that uses up to 100 color-coded bead sets, each of which can be conjugated with a different specific reactant. Thus, up to 100 different species can be simultaneously measured in a single tube or microplate well. LUMINEX assays involve the interaction of immobilized “bead-bound” capture molecules with a reaction partner (analyte) in solution. A reporter molecule, specific for the analyte, is used to quantify the interaction. The constituents of each well are drawn up into the flow-based LUMINEX array reader (Luminex 100/200), where each reaction (bead set) is identified by its spectral signature after excitation by the red classification laser. The attendant reporter signal from each reaction is simultaneously quantified by fluorescence generated by the green reporter laser. The Luminex software provides automated data analysis and the generation of detailed summary reports.


Benefits using Luminex

  • Small sample size—saves precious samples
  • Very sensitive—measurements are physiologically relevant
  • Short assay time—save time and labor expenses
  • High precision—ensure accurate results
  • Low background—no false negatives
  • Easy to use—special training not required
  • NIBSC/WHO calibrated standards, when available, and benchmarked to BioSource™ ELISA kits— protocol and data comparable to ELISA
  • Validated for serum, plasma, TCS, cell extracts, nuclear extracts—means less work for you!
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